Cyclin Dependent Kinase 1 Phospho-Thr14 (CDK1 pT14) Antibody
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Description
Rabbit polyclonal antibody against CDK1 protein. Immunogen region is N-terminal. Specificity is as follows for the reactive species: H:T14, M:T14, R:T14.
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Product specifications
| Category | Primary Antibodies |
| Immunogen Target | Cyclin Dependent Kinase 1 Phospho-Thr14 (CDK1 pT14) |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Assay Data | Modification: Phosphorylation // Target Modification: Thr14 |
| Recommended Dilution | ELISA: 1/1000, WB: 1/500 - 1/3000, IF/ICC: 1/100 - 1/500. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Purification | Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
| Size 1 | 10 µg |
| Size 2 | 100 µg |
| Size 3 | 200 µg |
| Size 4 | 300 µg |
| Size 5 | 1 mg |
| Form | Liquid |
| Tested Applications | ELISA, WB, IF/ICC |
| Buffer | PBS (without Mg<sup>2+</sup> and Ca<sup>2+</sup>), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P06493, P24941, Q00526 |
| Background | Antibody anti-CDK1 |
| Status | RUO |
| Note | Concentration: 1 mg/ml - |
Descripción
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The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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