Human Apolipoprotein L (APOL1) Protein

Product specifications

Proteins and Peptides

Apolipoprotein L (APOL1)

E. coli

Activity: Not tested
Sequence Fragment: Met1-Trp15+Val34-Ala238
Tag: N-terminal His tag and GST tag

Human

Unconjugated

Calculated MW: 54.2 kDa  Observed MW (SDS-PAGE): 56 kDa

Recombinant

> 95%

10 µg

50 µg

100 µg

200 µg

500 µg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

O14791

APOL1, APO-L, APOL, APOL-I, FSGS4, apolipoprotein L1, APO L1

Protein APOL1

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Endotoxin Level: <1.0 EU per 1µg (determined by the LAL method)
Concentration: Prior to lyophilization: 400 µg/ml

Descripción

Apolipoprotein L1 (APOL1) is a member of the Apolipoprotein L gene family and plays a critical role in lipid transport and innate immunity. APOL1 is primarily expressed in the liver, kidney, and vascular endothelium and is known for its ability to bind high-density lipoprotein (HDL) particles, contributing to lipid transport and cholesterol metabolism. A particularly vital function of APOL1 lies in its role in innate immunity, as it helps protect against trypanosome infections. APOL1 can lyse trypanosomes such as Trypanosoma brucei, which cause African sleeping sickness, by disrupting their cellular membranes. Genetic variations, specifically the G1 and G2 risk alleles, have been associated with increased susceptibility to kidney diseases, such as focal segmental glomerulosclerosis (FSGS) and HIV-associated nephropathy (HIVAN), particularly in people of African descent. These alleles enhance APOL1-mediated trypanosome resistance but lead to cytotoxic effects in kidney cells under specific stress conditions. APOL1’s dual role as both a defender against pathogens and a contributor to renal disease makes it a subject of intensive research. Targeting APOL1 signaling pathways may provide therapeutic options for managing kidney diseases and understanding its broader impact on lipid metabolism and immune responses.

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