Human Collagen Type I Alpha 1 (COL1A1) Protein

Proteins and Peptides

Collagen Type I Alpha 1 (COL1A1)

293F cell

Human

Unconjugated

Molecular Weight: Calculated MW: 21.0 kDa  Observed MW (SDS-PAGE): 38 kDa  Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.

Concentration: Prior to lyophilization: 80 µg/ml

Sequence Fragment: Arg253-Ala471

Tag: N-terminal His tag

Recombinant

> 80%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/ml. Do not vortex.

WB, SDS-PAGE

Prior to lyophilization: PBS, pH 7.4, containing 5% Trehalose.

Shipped within 5-7 working days.

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.

No

P02452

1277

Protein COL1A1

This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.