Human Growth Factor Receptor Bound Protein 2 (Grb2) Protein
702€ (50 µg)
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Name
Human Growth Factor Receptor Bound Protein 2 (Grb2) Protein
Category
Proteins and Peptides
Provider
Abbexa
Reference
abx655655
Tested Applications
WB, SDS-PAGE
Description
Human Growth Factor Receptor Bound Protein 2 (Grb2) Protein (Active) is an Active recombinant Human protein.
Documentos del producto
Instrucciones
Data sheet
Especificaciones del producto
| Category | Proteins and Peptides |
| Immunogen Target | Growth Factor Receptor Bound Protein 2 (Grb2) |
| Assay Type | Activity: Active Sequence Fragment: Please enquire. Tag: N-terminal His tag |
| Origin | Human |
| Conjugation | Unconjugated |
| Expression | Recombinant |
| Purity | > 90% |
| Size 1 | 50 µg |
| Size 2 | 100 µg |
| Size 3 | 200 µg |
| Size 4 | 500 µg |
| Size 5 | 1 mg |
| Form | Lyophilized |
| Tested Applications | WB, SDS-PAGE |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300. |
| Availability | Shipped within 5-7 working days. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Dry Ice | No |
| Alias | ASH, EGFRBP-Grb3-3, MST084, MSTP084, NCKAP2 |
| Background | Protein GRB2 |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. Concentration: Prior to lyophilization: 200 µg/ml |
Background
GRB2 is an adaptor protein that links receptor tyrosine kinases (RTKs) to downstream signaling pathways, including Ras/MAPK and PI3K/Akt. It consists of one SH2 domain flanked by two SH3 domains, enabling it to interact with phosphorylated tyrosines and proline-rich regions of target proteins. GRB2 primarily binds to proteins like SOS (a Ras GEF), Shc, and PLCγ, facilitating Ras activation and subsequent signaling for cell growth, proliferation, and differentiation. GRB2 is essential for embryonic development, cellular homeostasis, and immune responses. Dysregulation of GRB2 contributes to oncogenesis, particularly in breast, lung, and gastric cancers, where it enhances cell proliferation and survival through constitutive Ras signaling. GRB2 also plays roles in immune cell signaling, particularly T-cell receptor activation and cytokine production. Knockout models demonstrate embryonic lethality, highlighting its critical function in cellular signaling and development.
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