Human Interferon Beta (IFNB1) Protein

Proteins and Peptides

Interferon Beta (IFNB1)

E. coli

Activity: Active
Biological Activity: A proliferation assay was conducted to detect the bioactivity of recombinant human IFNb using A549 cells. Briefly, A549 cells were seeded into triplicate wells in 96-well plates at a density of 2,000 cells/well and allowed to attach with serum-free medium overnight, then the medium was replaced with 5% serum standard DMEM prior to the addition of various concentrations of IFNb. After incubation for 96 h, cells were observed by inverted microscope (Figure 3) and cell proliferation was measured by Cell Counting Kit-8 (CCK-8) assay (Figure 4). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C. The ED50 of recombinant human IFNb is 6.4 µg/ml.
Sequence Fragment: Met22-Asn187
Tag: N-terminal His tag

Human

Unconjugated

Calculated MW: 23.7 kDa  Observed MW (SDS-PAGE): 25 kDa

Recombinant

> 80%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

P01574

3456

Protein IFNB1

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Reconstitute in ddH2O to a concentration between 0.1-0.5 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU per 1 μg (LAL method)
Concentration: Prior to lyophilization: 100 µg/ml