Human Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) ELISA Kit

Este producto es parte de SIGLEC - sialic acid binding Ig like lectin
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585€ (96 tests)

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935106861
info@markelab.com
name
Human Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) ELISA Kit
category
ELISA Kits
provider
Abbexa
reference
abx253175
tested applications
ELISA

Description

Human Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human Sialic Acid Binding Immunoglobulin Like Lectin 8 concentrations in tissue homogenates, cell lysates and other biological fluids.

Documents del producto

Instrucciones
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Data sheet
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Product specifications

Category
ELISA Kits
Immunogen Target
Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8)
Reactivity
Human
Detection Method
Colorimetric
Assay Data
Quantitative
Assay Type
Sandwich
Test Range
0.156 ng/ml - 10 ng/ml
Sensitivity
0.1 ng/ml
Recommended Dilution
Optimal dilutions/concentrations should be determined by the end user.
Size 1
96 tests
Size 2
5 × 96 tests
Size 3
10 × 96 tests
Form
Standard Form: Lyophilized
Tested Applications
ELISA
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Availability
Shipped within 5-7 working days.
Storage
Shipped at 4°C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Dry Ice
No
UniProt ID
Q9NYZ4
Alias
SAF2,SIGLEC-8,SIGLEC8L,Sialoadhesin family member 2
Background
Elisa Kits for: SIGLEC8
Status
RUO
Note
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Descripción

SIGLEC8 is an inhibitory receptor expressed mainly on eosinophils, mast cells, and basophils. It binds selectively to 6′-sulfated sialyl Lewis X and related glycan ligands, regulating inflammatory responses in allergic diseases. Activation of SIGLEC8 leads to eosinophil apoptosis and inhibition of mast cell degranulation, making it an attractive therapeutic target for asthma and eosinophilic disorders. Its function is mediated through ITIMs, which dampen cell activation pathways. SIGLEC8 is also implicated in maintaining immune homeostasis in tissues and resolving allergic inflammation.

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SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2, 3-linked sialic acid. and also binds to alpha2, 6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.

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