Human T-lymphocyte activation antigen CD80 / B7-1 (CD80) Protein

Proteins and Peptides

T-lymphocyte activation antigen CD80 / B7-1 (Anti-CD80)

293F cells

Activity: Active
Biological Activity: EC50: 0.412 µg/ml
A functional binding ELISA assay was conducted to detect the interaction of recombinant Human CD80 with recombinant human CD86. Briefly, serial dilutions of recombinant rat Human T-lymphocyte activation antigen CD80 / B7-1 (CD80) Protein were carried out in PBS, 0.01 % BSA (pH 7.4). Duplicate samples of 100 µl were transferred to microtiter wells pre-coated with CD86 and incubated for 1h at 37 °C. Wells were washed with PBST 3 times and incubated with Streptavidin-HRP for 40 minutes, then aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 °C. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. 
Sequence Fragment: Val35-Asn242
Tag: N-terminal His tag

Human

Unconjugated

Calculated MW: 25.5kDa
Observed MW (SDS-PAGE): 40-60 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.

Recombinant

> 95%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

ELISA

Prior to lyophilization: PBS, pH 7.4, containing 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

P33681

B7,BB1,B7-1,B7.1,LAB7,CD28LG,CD28LG1,Activation B7-1 antigen,CTLA-4 counter-receptor B7.1,T-lymphocyte activation antigen CD80

Protein CD80

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU/µg (determined by the LAL method)
Concentration: Prior to lyophilization: 1100 µg/ml