Pig Interferon Beta (IFNB1) Protein

Proteins and Peptides

Interferon Beta (IFNB1)

E. coli

Activity: Active
Biological Activity: Interferon Beta (IFNb) is a member of type I interferons (IFNs) family which is a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNb proteins are produced in large quantities by fibroblasts. They have antiviral activity that is involved mainly in innate immune response. Two types of IFNb have been described, IFNb1 (IFNB1) and IFNb3 (IFNB3). IFNb1 is used as a treatment for multiple sclerosis as it reduces the relapse rate. Besides, Interferon Alpha/Beta Receptor 1 (IFNa/bR1) has been identified as an interactor of IFNb, thus a binding ELISA assay was conducted to detect the interaction of recombinant Pig IFNb and recombinant Pig IFNa/bR1. Briefly, IFNb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to IFNa/bR1-coated microtiter wells and incubated for 2 h at 37 °C .Wells were washed with PBST and incubated for 1 h with anti-IFNb polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 min at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. 
Sequence Fragment: Met22-Asn186
Tag: N-terminal His tag and GST tag

Pig

Unconjugated

Calculated MW: 49.62 kDa  Observed MW (SDS-PAGE): 50 kDa

Recombinant

> 98%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl and 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

Q29412

IFB,IFF,IFNB,IFN-beta,IFN-beta 1,Fibroblast interferon 1,IFNB1

Protein IFNB1

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Reconstitute in ddH2O to a concentration between 0.1-0.5 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU per 1 μg (LAL method)
Concentration: Prior to lyophilization: 50 µg/ml