Rabbit Interleukin 8 / IL8 (CXCL8) Protein
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Description
Rabbit Interleukin 8 / IL8 (CXCL8) Protein is an active recombinant protein, expressed in E. coli.
Documents del producto
Product specifications
| Category | Proteins and Peptides |
| Immunogen Target | Interleukin 8 / IL8 (CXCL8) |
| Host | E. coli |
| Assay Type | Activity: Active Biological Activity: A microchemotaxis assay was a carried out to determine the chemotactic effect of IL8 on Jurkat cells. 100 µm cell suspension (1 × 106 cells per ml) in RPMI 1640 FBS free medium were seeded to the upper chamber of a microchemotaxis system and Human IL8 (0.1 ng/ml, 1 ng/ml and 10 ng/ml dilutions in RPMI 1640) was added to the lower chamber with an 8 µm polycarbonate filter to separate the two compartments. The cells were incubated at 5% CO2, 37°C for 1 hour, the filter was removed and the cells in the lower chamber were observed at x100 magnification and counted at x400 magnification. It was determined that optimal chemotaxis of Jurkat cells occurs at 1-10 ng/ml concentrations of Human IL8. Sequence Fragment: Ala23-Ser101 Tag: N-terminal His tag |
| Origin | Rabbit |
| Conjugation | Unconjugated |
| Observed MW | Calculated MW: 12.5 kDa Observed MW (SDS-PAGE): 14 kDa |
| Expression | Recombinant |
| Purity | > 95% |
| Size 1 | 50 µg |
| Size 2 | 100 µg |
| Size 3 | 200 µg |
| Size 4 | 500 µg |
| Size 5 | 1 mg |
| Form | Lyophilized |
| Tested Applications | WB, SDS-PAGE |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
| Availability | Shipped within 5-7 working days. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Dry Ice | No |
| UniProt ID | P19874 |
| Gene ID | 100009129 |
| Alias | IL8,NAF,GCP1,LECT,LUCT,NAP1,GCP-1,LYNAP,MDNCF,MONAP,NAP-1,SCYB8,C-X-C motif chemokine 8,Emoctakin,Granulocyte chemotactic protein 1,Monocyte-derived neutrophil chemotactic factor |
| Background | Protein CXCL8 |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. Endotoxin Level: < 1.0 EU per 1 µg (LAL method). Concentration: Prior to lyophilization: 400 µg/ml |
Descripción
CXCL8, also known as Interleukin-8 (IL-8), is a pro-inflammatory chemokine belonging to the CXC chemokine family. It plays a critical role in immune responses by promoting the recruitment and activation of neutrophils, which are essential in the body’s response to infection and injury. CXCL8 is secreted by a wide range of cells, including macrophages, epithelial cells, endothelial cells, fibroblasts, and keratinocytes, particularly in response to inflammatory stimuli like bacterial products, oxidative stress, and pro-inflammatory cytokines such as IL-1 and TNF-α. CXCL8 is a potent chemoattractant that directs the migration of neutrophils from the bloodstream to the site of infection or tissue damage. This chemotactic activity is critical for the rapid recruitment of neutrophils, allowing them to carry out their functions in pathogen destruction and the removal of damaged cells.
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