Rat Intestinal-Type Alkaline Phosphatase / IAP (ALPI) CLIA Kit

Este producto es parte de ALPI - Intestinal-type alkaline phosphatase
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845€ (96 tests)

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935106861
info@markelab.com
name
Rat Intestinal-Type Alkaline Phosphatase / IAP (ALPI) CLIA Kit
category
CLIA Kits
provider
Abbexa
reference
abx492401
tested applications
CLIA

Description

Rat Intestinal-Type Alkaline Phosphatase / IAP (ALPI) Chemiluminescent Immunoassay (CLIA) Kit is a Sandwich Chemiluminescent Immunoassay (CLIA) Kit for use with Serum, plasma, tissue homogenates and other biological fluids.

Documents del producto

Instrucciones
Data sheet
Descargar

Product specifications

Category
CLIA Kits
Immunogen Target
Intestinal-Type Alkaline Phosphatase / IAP (ALPI)
Reactivity
Rat
Detection Method
Chemiluminescent
Assay Data
Quantitative
Assay Type
Sandwich
Test Range
0.14 ng/ml - 100 ng/ml
Sensitivity
< 0.05 ng/ml
Recommended Dilution
Optimal dilutions/concentrations should be determined by the end user.
Size 1
96 tests
Size 2
5 × 96 tests
Size 3
10 × 96 tests
Form
Standard: Lyophilized
Tested Applications
CLIA
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Availability
Shipped within 5-20 working days.
Storage
Shipped at 4°C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Dry Ice
No
Alias
IAP
Background
CLIA Kits ALPI
Status
RUO
Note
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES.  The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.  Please note that our kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Descripción

Intestinal-type alkaline phosphatase (ALPI) is a glycoprotein enzyme primarily expressed on the luminal surface of intestinal epithelial cells, particularly in the small intestine. It plays a crucial role in maintaining gut homeostasis by detoxifying lipopolysaccharides (LPS), dephosphorylating inflammatory mediators, and promoting a healthy microbiota. ALPI is also involved in nutrient absorption, such as the hydrolysis of dietary phosphate esters, contributing to the regulation of phosphate metabolism. Its expression is upregulated in response to inflammation and microbial imbalance, acting as a protective mechanism against intestinal permeability and systemic endotoxemia. Additionally, ALPI prevents excessive immune activation by neutralizing pro-inflammatory signals, which is essential for preventing conditions such as inflammatory bowel disease (IBD) and sepsis. Alterations in ALPI expression or activity have been linked to gastrointestinal disorders, including Crohn's disease and ulcerative colitis. Its multifunctional role highlights its importance in gut health, immune modulation, and overall metabolic regulation.

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