Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Antibody
260€ (100 µl)
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Name
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Antibody
Category
Primary Antibodies
Provider
Abbexa
Reference
abx005569
Tested Applications
ELISA, WB, IHC, IF/ICC
Description
GAPDH Antibody is a Mouse Monoclonal antibody against GAPDH. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocated to the nucleus post modification such as S-nitrosylation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Documentos del producto
Instrucciones
Data sheet
Especificaciones del producto
| Category | Primary Antibodies |
| Immunogen Target | Target: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH. |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Assay Type | Concentration: 2.91 mg/ml |
| Recommended Dilution | ELISA: 1 µg/ml, WB: 1/5000 - 1/50000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Monoclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Observed MW | Calculated MW: 36 kDa Observed MW: 36 kDa |
| Purification | Purified by affinity chromatography. |
| Size 1 | 100 µl |
| Size 2 | 2 × 100 µl |
| Form | Liquid |
| Tested Applications | ELISA, WB, IHC, IF/ICC |
| Buffer | PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P04406 |
| Gene ID | 2597 |
| Alias | GAPDH, GAPD, G3PD, HEL-S-162eP,Peptidyl-cysteine S-nitrosylase GAPDH |
| Background | Antibody anti-GAPDH |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional enzyme predominantly involved in glycolysis, catalyzing the oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate using NAD+ as a cofactor. Beyond its metabolic role, GAPDH participates in various non-metabolic processes, including transcriptional regulation, DNA repair, and cell apoptosis. Its involvement in oxidative stress response and as a target for S-nitrosylation connects it to neurodegenerative diseases such as Alzheimer's and Parkinson's. Furthermore, GAPDH can translocate to the nucleus under stress conditions, influencing apoptotic pathways.
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