Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Antibody
299€ (50 µl)
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Name
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Antibody
Category
Primary Antibodies
Provider
Abbexa
Reference
abx349907
Tested Applications
ELISA, WB, IHC, IF/ICC, FCM
Description
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Antibody is a Recombinant Rabbit Monoclonal antibody for the detection of Human GAPDH.
Documentos del producto
Instrucciones
Data sheet
Especificaciones del producto
| Category | Primary Antibodies |
| Immunogen Target | Target: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Immunogen: Recombinant Human GAPDH protein |
| Host | Rabbit |
| Reactivity | Human |
| Recommended Dilution | WB: 1/1000 - 1/5000, IHC: 1/20 - 1/200, IF/ICC: 1/20 - 1/200, FCM: 1/20 - 1/200. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Monoclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Expression | Recombinant |
| Purification | Purified by affinity chromatography. |
| Size 1 | 50 µl |
| Size 2 | 100 µl |
| Form | Liquid |
| Tested Applications | ELISA, WB, IHC, IF/ICC, FCM |
| Buffer | 0.01 M PBS, pH 7.4, 0.03% Proclin-300 and 50% glycerol. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P04406 |
| Alias | GAPDH, GAPD, G3PD, HEL-S-162eP,Peptidyl-cysteine S-nitrosylase GAPDH |
| Background | Antibody anti-GAPDH |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional enzyme predominantly involved in glycolysis, catalyzing the oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate using NAD+ as a cofactor. Beyond its metabolic role, GAPDH participates in various non-metabolic processes, including transcriptional regulation, DNA repair, and cell apoptosis. Its involvement in oxidative stress response and as a target for S-nitrosylation connects it to neurodegenerative diseases such as Alzheimer's and Parkinson's. Furthermore, GAPDH can translocate to the nucleus under stress conditions, influencing apoptotic pathways.
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