Mouse A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) Protein

Proteins and Peptides

A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7)

E. coli

Activity: Active
Biological Activity: Mouse A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) Protein (Active), is a secreted zinc-dependent metalloproteinase belonging to the ADAMTS family, characterized by an N-terminal metalloproteinase domain, disintegrin-like module, and multiple thrombospondin type 1 repeats. Primarily expressed in cardiovascular tissues, skeletal muscle, and cartilage, ADAMTS7 exerts proteolytic functions by cleaving extracellular matrix (ECM) components, such as aggrecan and versican, thereby regulating ECM remodeling. Emerging evidence highlights its critical role in atherosclerosis: it promotes vascular smooth muscle cell (VSMC) migration and proliferation, accelerates plaque formation, and correlates with increased cardiovascular disease risk. Additionally, ADAMTS7 participates in osteoarthritis pathogenesis by degrading cartilage matrix proteins. As a multifunctional protease, it serves as a potential therapeutic target for cardiovascular and musculoskeletal disorders. ADAMTS7 interacts with COMP, likely forming a complex to modulate ECM homeostasis and tissue remodeling processes.Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse ADAMTS7 and recombinant rat COMP. Briefly, ADAMTS7 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to COMP-coated microplate wells and incubated for 1 h at 37℃. Wells were washed with PBST and incubated for 1 h with anti-ADAMTS7 polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody for 1 h at 37℃, wells were aspirated and washed 5 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37℃. Finally, 50 μl stop solution was added to the wells and absorbance was read at 450 nm immediately. The binding activity of ADAMTS7 and COMP is shown in Figure 3. The EC50 for this effect is 0.193 µg/ml.
Sequence Fragment: Lys226-Pro437
Tag: N-terminal His tag

Mouse

Unconjugated

Calculated MW: 27.7 kDa  Observed MW (SDS-PAGE): 27 kDa

Recombinant

> 90%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl and 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

Q68SA9

Protein ADAMTS7

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU per 1 µg (determined by the LAL method)
Concentration: Prior to lyophilization: 200 µg/ml