Recombinant Human SHMT1
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935106861
info@markelab.com
name
Recombinant Human SHMT1
category
Proteins and Peptides
provider
FineTest
reference
P5106
tested applications
Western Blot, ELISA
Documents del producto
Instrucciones
Data sheet
Product specifications
| Category | Proteins and Peptides |
| Host | E.Coli |
| Reactivity | Human |
| Assay Data | Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex. |
| Recommended Dilution | ¥ |
| Isotype | ¥ |
| Clone ID | ¥ |
| Observed MW | 53 kDa |
| Expression | 1-483 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Size 1 | 50μg |
| Size 2 | 200μg |
| Size 3 | 1mg |
| Form | Lyophilized powder |
| Tested Applications | Western Blot, ELISA |
| Buffer | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4. |
| Availability | 3-4 weeks |
| Storage | The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle. |
| UniProt ID | P34896 |
| Alias | CSHMT, Cytoplasmic serine hydroxymethyltransferase, cytosolic, GLYC_HUMAN, Glycine hydroxymethyltransferase, Serine hydroxymethyltransferase, Serine hydroxymethyltransferase 1 (soluble), Serine hydroxymethyltransferase, cytosolic, Serine methylase, SHMT, Shmt1 |
| Background | Protein SHMT1 |
| Status | RUO |
| Note | Tag : N-terminal His Tag or N-terminal His-IF2DI Tag, determined during production process |
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This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5, 10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants.
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Recombinant Human SHMT1
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