Recombinant Human SHMT1
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935106861
info@markelab.com
name
Recombinant Human SHMT1
category
Proteins and Peptides
provider
FineTest
reference
Pr22065
tested applications
Western Blot, ELISA
Documents del producto
Instrucciones
Data sheet
Product specifications
| Category | Proteins and Peptides |
| Host | Mammalian Cells |
| Reactivity | Human |
| Assay Data | Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex. |
| Recommended Dilution | ¥ |
| Isotype | ¥ |
| Clone ID | ¥ |
| Observed MW | 55 kDa |
| Expression | Met3-Phe483 |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Size 1 | 10μg |
| Size 2 | 50μg |
| Size 3 | 500μg |
| Size 4 | 1mg |
| Form | Lyophilized powder |
| Tested Applications | Western Blot, ELISA |
| Buffer | Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM EDTA, 5% Trehalose, 5% Mannitol, 0.02% Tween80, pH 6.0. |
| Availability | 7 days |
| Storage | The lyophilized protein is stable at -20°C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80°C for 3 months or 1 week at 2 to 8°C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle. |
| UniProt ID | AAH07979.1 |
| Alias | Serine Hydroxymethyltransferase Cytosolic, SHMT, Glycine Hydroxymethyltransferase, Serine Methylase, SHMT1 |
| Background | Protein SHMT1 |
| Status | RUO |
| Note | Endotoxin: < 1 EU/µg as determined by LAL test. Tag : C-terminal His Tag |
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This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5, 10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants.
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Recombinant Human SHMT1
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