XmaI Enzyme

Proteins and Peptides

XmaI

E. coli

Activity: Active
Biological Activity: One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37 °C in a total reaction volume of 50 µl.  Recognition site:  5’...C↓CCGGG...3’  3’...GGGCC↑C...5’

37.6 kDa

250 U

500 U

Storage Buffer: 20 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1.5 mM DTT, 0.4 mg/ml BSA, 50% glycerol. <br> 10X Buffer: 100 mM Tris-Ac (pH 7.9), 1 M KAc, 120 mM MgAc<sub>2</sub>, 1 mg/ml BSA.

Shipped within 10-20 working days.

Store at -20 °C. Stable for up to 2 years from date of receipt.

Yes

Protein XmaI Enzyme

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.  This product is shipped with dry ice.
Thaw the 10X Buffer and bring to room temperature before use. For ≤ 0.5 µg DNA, add 2 µl of 10X Buffer, 0.5 µl of XmaI, and nuclease-free water (variable volume) to the sample to make a final reaction system volume of 20 µl. For ≤ 1 µg DNA, add 5 µl of 10X Buffer, 1 µl of XmaI, and nuclease-free water (variable volume) to the sample to make a final reaction system volume of 50 µl. For > 2 µg DNA or incomplete digestion, increase the volume of enzyme. The total volume of enzyme should be less than 1/10 of the volume of the reaction system.

Incubate for 5-15 minutes at 37 °C. Inactivate the enzyme by adding 10X DNA Loading Buffer to a final concentration of 1X, or by heating at 65 °C for 20 minutes.

Note: Low ionic strength, high enzyme concentration, glycerol concentration > 5% or pH > 8.0 may result in star activity.